Everything about Eukaryotic Gene Example totally explained
Many
genomes have been
sequenced and their gene sequences are stored in general DNA sequence databases (for example
GenBank) and in species specific databases (for example The
Arabidopsis Information Resource (TAIR).
The figures are views of the sequence of one (AMY1) of the approximately 25,000 genes from
Arabidopsis thaliana the
Thale Cress plant.
This gene encodes an alpha
amylase enzyme.
These images are views of the gene, cDNA, and coding sequence (
CDS) used by researchers to study it further. Such research might involve seed germination or plant flavour.
cDNA
The image below shows a screenshot of the
AMY1
cDNA. This was obtained from
TAIR
Note that TAIR provide three views of the 'Nucleotide Sequence', 'full length CDS', 'full length cDNA' (Fig. 1), 'full length genomic' (Fig. 2]. In each of them the DNA alphabet is used, although strictly the CDS should be shown as RNA (AUG etc).
A typical eukaryotic gene is transcribed into an
pre-mRNA that's then processed into a mature
mRNA by removal of
introns and 5' and 3' processing.
Gene and mRNA
The mature
mRNA is comprised of a
5' UTR (red)
CDS (uppercase yellow) and
3' UTR (red again) (Fig. 2). All three of these regions are
exonic (not just the protein coding sequence (CDS)).
Introns are shown in purple (lowercase) and are not present in the mature mRNA.
For convenience neither the 5' Cap nor 3' tail are shown in the cDNA (fig 1) although the mRNA will have them. The gene sequence is also shown in a form where the codons can be read (ATG...), rather than as the
template DNA strand which is actually copied into mRNA.
This gene structure view is typical of a eukaryotic gene. Similar views of genes can be obtained for
human,
fruitfly, or
yeast genes.
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